Bioethanol (biofuel) Production from Tea Waste: A Comparative Study Using Cellulase enzyme, Baker’s Yeast, and Wild Saccharomyces Cerevisiae Cultivated from Rice bran in the context of Bangladesh
DOI:
https://doi.org/10.38032/scse.2026.4.170Keywords:
Tea Waste, Bioethanol, Saccharomyces cerevisiae, Enzymatic Hydrolysis, Waste Valorization.Abstract
Bangladesh ranks among the top twenty tea-producing nations, producing over 95 million kg of tea in 2021. This large-scale production generates considerable tea waste—infused leaves, branches, and discarded leaves posing environmental and economic challenges. Tea residues are abundant in cellulose, hemicellulose, lignin, and bioactive substances. This study was designed to evaluate three bioconversion routes like fermentation using wild Saccharomyces cerevisiae cultured from rice bran, commercial baker’s yeast, and cellulase-mediated enzymatic hydrolysis methods. Bioethanol concentration (%v/v) from all the three media was measured using a double beam UV-Visible Spectrophotometer by absorption method. Enzymatic saccharification fermentation showed the highest ethanol yield with 4.10(%v/v) in cellulase enzyme containing treated samples, followed by wild yeast fermentation with 3.12(%v/v) in untreated samples. In case of commercial yeast extract, fermentation yield was 2.64(%v/v) in treated samples. The above results demonstrate that tea waste is available raw material for bioethanol production. The adoption of such processes could contribute to renewable energy regeneration and waste valorization in Bangladesh.
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Copyright (c) 2026 Ashikur Rahman Khan Asik , Zannatul Nayem Showmik , Mohammad Nurur Rahman , Mohammad Moffajjel Hossain , Md. Masumul Haque , Md. Faruk Hasan , Aktarun Nahar , Runa Afroz Rintu (Author)
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