Biogenic Synthesis of Ni-doped Iron Oxide (Fe_2 O_3) Nanoparticles from Hibiscus Sabdariffa (Rosella) Leaf Extract and Investigating their Antibacterial Activity
DOI:
https://doi.org/10.38032/scse.2025.3.33Keywords:
Biogenic Synthesis, Iron oxide nanoparticles, Rosella leaf, Hibiscus Sabdariffa, Antibacterial activityAbstract
Iron oxide ( ) nanoparticles have various types of applications and in this era, biogenic synthesis is the most effective and eco-friendly method to synthesize the nanoparticles. This research aimed to synthesize the Ni-doped Iron oxide nanoparticles by using leaf extract of Hibiscus Sabdariffa (Rosella), Iron (III) nitrate nonahydrate as the source of Iron, and Nickle nitrate hexahydrate as the source of Nickle. The synthesized nanoparticles were analyzed through various techniques such as X-ray diffraction (XRD), scanning electron microscopy (SEM), and UV-visible spectroscopy to determine their structural, morphological, and optical properties. XRD result of NPs confirmed the formation of crystalline iron oxide NPs and Ni-doped iron oxide NPs with the average crystalline size of 13.90 nm and 14.94 nm respectively. SEM analysis provided NPs insights into the surface morphology and particle size, revealing spherical nanoparticles size between 10 nm to 90 nm, with average spherical size of iron oxide NPs and Ni-doped NPs is 46.13 nm ± 4.71 nm and 35.27 nm ± 1.32 nm respectively. UV visible spectroscopy analysis showed the absorbance and energy band gap of both nanoparticles. Antibacterial activity revealed variations in the zone of inhibition between pure iron oxide NPs and Ni-doped iron oxide NPs. This biogenic method is not only eco-friendly but also cost-effective with its various types of potential applications.
Downloads
Downloads
Downloads
References
[1] Singh Y, Kaur A, Suri B and Singhal S. “Systematic literature review on regression test prioritization techniques”, Informatica, Vol. 36, pp. 379–408, 2012.
[2] Hafeez, M., Shaheen, R., Akram, B., Zain-Ul-Abdin, N., Haq, S., Mahsud, S., Ali, S., & Khan, R. T.” Green synthesis of cobalt oxide nanoparticles for potential biological applications.” Materials Research Express, Vol.7, No. 2, 2020.
[3] Buzea, C., Pacheco, I. I., & Robbie, K., “Nanomaterials and nanoparticles: Sources and toxicity”, Biointerphases, Vol. 2, No.4, MR17–MR71, 2017.
[4] Athawale A A, Majumdar M, Singh H and Navinkiran K 2010 Synthesis of cobalt oxide nanoparticles/fibres in alcoholic medium using y-ray technique Def. S. J. 60 507–13.
[5] Priya, N., Naveen, N., Kaur, K., & Sidhu, A. K. “Green Synthesis: An Eco-friendly Route for the Synthesis of Iron Oxide Nanoparticles.” Frontiers in Nanotechnology, Vol. 3, 2021
[6] Ali Talha Khalil, Muhammad Ovais, Ikram Ullah, Muhammad Ali, Zabta Khan Shinwari, Malik Maaza, “Biosynthesis of iron oxide (Fe2O3) nanoparticles via aqueous extracts of Sageretia thea (Osbeck.) and their pharmacognostic properties”, Green Chemistry Letters and Reviews , Vol. 10, No. 4, pp. 186–201, 2017.
[7] Nasrin Beheshtkhoo, Mohammad Amin Jadidi Kouhbanani, Amir Savardashtaki, Ali Mohammad Amani, Saeed Taghizadeh, “Green synthesis of iron oxide nanoparticles by aqueous leaf extract of Daphne mezereum as a novel dye removing material”, Applied Physics A, Vol. 124, No. 5, 2018.
[8] P. Durga Sruthi, Chamarthy Sai Sahithya, C. Justin, C. SaiPriya, Karanam Sai Bhavya, P. Senthilkumar and Antony V. Samrot, “Utilization of Chemically Synthesized Super Paramagnetic Iron Oxide Nanoparticles in Drug Delivery, Imaging and Heavy Metal Removal”, Journal of Cluster Science, Vol. 30, No. 1, pp. 11–24, 2019.
[9] Zhang, R., Zhou, Y., Yan, X., & Fan, K, “Advances in chiral nanozymes: a review”, Microchimica Acta, Vol. 186, No. 782, pp. 1-12, 2019.
[10] Md. Shakhawat Hossen Bhuiyan, Muhammed, Yusuf Miah, Shujit Chandra Paul, Tutun Das Aka, Otun Saha, Md. Mizanur Rahaman, Md. Jahidul Islam Sharif, Ommay Habiba and Md. Ashaduzzaman, “Green synthesis of iron oxide nanoparticle using Carica papaya leaf extract: application for photocatalytic degradation of remazol yellow RR dye and antibacterial activity”, Heliyon, Vol. 6, No. 8, p. e04603, 2020.
[11] Gao, L., Fan, K., Yan, X., Iron Oxide Nanozyme: A Multifunctional Enzyme Mimetics for Biomedical Application. In: Yan, X. (eds) Nanozymology. Nanostructure Science and Technology. Springer, Singapore, pp. 105-140, 2020.
[12] Malhotra, N., Lee, J.-S., Liman, R. A. D., Ruallo, J. M. S., Villaflores, O. B., Ger, T.-R., & Hsiao, C.-D., “Potential Toxicity of Iron Oxide Magnetic Nanoparticles: A Review”, Molecules, Vol. 25, Issue 14, p. 3159, 2020.
[13] Piyal Mondal, A. Anweshan, and Mihir Kumar Purkait, “Green synthesis and environmental application of iron-based nanomaterials and nanocomposite: A review”, Chemosphere,Vol. 259, p. 127509, 2020
[14] Vasantharaj, S., Sathiyavimal, S., Senthilkumar, P., LewisOscar, F., & Pugazhendhi, A., “Biosynthesis of iron oxide nanoparticles using leaf extract of Ruellia tuberosa: Antimicrobial properties and their applications in photocatalytic degradation”, Journal of Photochemistry and Photobiology B: Biology, Vol. 192, pp. 74–82, 2019.
[15] Tong, W., Hui, H., Shang, W., Zhang, Y., Tian, F., Ma, Q., Yang, X., Tian, J., & Chen, Y., “Highly sensitive magnetic particle imaging of vulnerable atherosclerotic plaque with active myeloperoxidase-targeted nanoparticles”, Theranostics, Vol. 11, No. 2, pp. 506–521, 2021.
[16] Moharana, A., Kumar, D., & Kumar, A., “Synthesis of Ni doped iron oxide nanoparticles and their dielectric properties”, Journal of Physics: Conference Series, Vol. 1531, No. 1, p. 012113, 2020.
[17] Francis Leonard Deepak, Manuel Bañobre-López, Enrique Carbó-Argibay, M. Fátima Cerqueira, Yolanda Piñeiro-Redondo, José Rivas, Corey M. Thompson, Saeed Kamali, Carlos Rodríguez-Abreu, Kirill Kovnir, and Yury V. Kolen’ko, “A Systematic Study of the Structural and Magnetic Properties of Mn-, Co-, and Ni-Doped Colloidal Magnetite Nanoparticles”, The Journal of Physical Chemistry C, Vol. 119, No. 21, pp.11947-11957, 2015.
[18] Yong-Sheng Hu, Alan Kleiman-Shwarsctein, Arnold J. Forman, Daniel Hazen, Jung-Nam Park, and Eric W. McFarland, “Pt-doped α-Fe2O3 thin films active for photoelectrochemical water splitting”, Chemistry of Materials , Vol. 20, No. 12, pp.3803–3805, 2008.
[19] S. Park, H.J. Kim, C.W. Lee, H.J. Song, S.S. Shin, S.W. Seo, H.K. Park, S. Lee, D.- W. Kim, K.S. Hong,“Sn self-doped -Fe2O3 nanobranch arrays supported on a transparent, conductive SnO2 trunk to improve photoelectrochemical water oxidation”, International Journal of Hydrogen Energy, Vol. 39, No. 29, pp.16459–16467, 2014.
[20] J. Frydrych, L. Machala, J. Tucek, K. Siskova, J. Filip, J. Pechousek, K. Safarova, M. Vondracek, J.H. Seo, O. Schneeweiss, M. Gr¨ Atzel, K. Sivula, R. Zboril, “Facile fabrication of tin-doped hematite photoelectrodes effect of doping on magnetic properties and performance for light-induced water splitting”, Journal of Materials Chemistry, Vol.22, No. 43, pp. 23232-23239 , 2012.
[21] J. Engel, H.L. Tuller, “The electrical conductivity of thin film donor doped hematite: from insulator to semiconductor by defect modulation”, Physical Chemistry Chemical Physics, Vol. 16, No. 23, pp. 11374–11380, 2014.
[22] Thakur, N., Kumar, P., Tapwal, A., & Jeet, K., “Degradation of malachite green dye by capping polyvinylpyrrolidone and Azadirachta indica in hematite phase of Ni doped Fe2O3 nanoparticles via co-precipitation method”, Nanofabrication, Vol. 8, 2023.
[23] Gattu, K. P., Ghule, K., Kashale, A. A., Patil, V. B., Phase, D. M., Mane, R. S., Han, S. H., Sharma, R., & Ghule, A. V., “Bio-green synthesis of Ni-doped tin oxide nanoparticles and its influence on gas sensing properties”, RSC Advances, Vol. 5, No. 89, pp. 72849–72856, 2015.
[24] Indryani Fauhan, K., Ehrich Lister, I. N., & Fachrial, E., “The Use of Rosella (Hibiscus sabdariffa L.) Extract Cream to Prevent Decreasing of Total Collagen in the Skin of Wistar Rats Exposed to Ultraviolet-B Light”, Journal of Pharmaceutical Research International, Vol. 35, No. 1, pp. 24–32, 2023.
[25] Vitri Nurilawaty, Tedi Purnama, Ai Emalia Sukmawati2, Silvester Maximus Tulandi, “The Potential of Rosella Floss (Hibiscus Sabdariffa l.) as a Dental Plaque Disclosing Agent”, Journal of International Dental and Medical Research, Vol. 16, No. 4, pp. 1454-1461, 2023.
[26] Fitriaturosidah, I., Kusnadi, J., Nurnasari, E., Nurindah, & Hariyono, B., “Phytochemical screening and chemical compound of green roselle(Hibiscus sabdariffa L.) and potential antibacterial activities”, IOP Conference Series: Earth and Environmental Science, Vol. 974, No. 1, p. 012118, 2022.
[27] Bauer, A.W., Kirby, W.M.M., Sherris, J.C., Truch, M., “Antibiotic susceptibility testing by standardized single disk method”, American Journal of Clinical Pathology, Vol. 45, No. 4, pp. 493–496, 1996.
[28] Asma Almontasser, Azra Parveen, “Probing the effect of Ni, Co and Fe doping concentrations on the antibacterial behaviors of MgO nanoparticles”, Scientific Reports, Vol. 12, No. 1, pp. 1-33, 2022.
[29] Deepthi, S., Vidya, Y. S., Manjunatha, H. C., Sridhar, K. N., Manjunatha, S., Munirathnam, R., Shivanna, M., kumar, S., & Ganesh, T., “Green photoluminescence, supercapacitor and cytotoxic properties of nickel doped haematite nanoparticles”, Chemical Physics Impact, Vol. 9, p. 100708, 2024.
[30] T. Kamakshi, G. Sunita Sundari, Harikrishna Erothu, Subhakaran Singh Rajaputra, “Effect of nickel dopant on structural, morphological and optical characteristics of Fe3O4 nanoparticles” Rasayan Journal of Chemistry, Vol. 12, No. 2, pp. 531-536, 2019.
[31] Velsankar, K., Parvathy, G., Mohandoss, S., Krishna Kumar, M., & Sudhahar, S., “Celosia argentea leaf extract-mediated green synthesized iron oxide nanoparticles for bio-applications”, Journal of Nanostructure in Chemistry, Vol. 12, No. 4, pp. 625–640, 2021.
Published
Conference Proceedings Volume
Section
License
Copyright (c) 2025 Md. Lael Hasan, Hridoy Roy, Md. Estabrak Ahammod Sakib, Md. Farhan Muskan (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
All the articles published by this journal are licensed under a Creative Commons Attribution 4.0 International License
